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The ancestral character reconstructions (genome size, number of rDNA sites and number of CMA+ bands) were conducted using unordered maximum parsimony as implemented for continuous and meristic characters in Mesquite v. 3.02 software [ 103] using the 50%% majority-rule consensus tree resulting from the Bayesian inference analysis as the input tree file.
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Immunoblotting images presented here are representative of three experiments, and densitometric evaluation of the bands was conducted using UN-SCAN-IT gel densitizing software (Silk Scientific, Orem, UT).
Quantification of Western blot bands was conducted using Image J. Lymphoblastoid cell lines from 4 female controls and 4 female patients with high XIST expression were selected for examination of H3K27me3 on the inactive X chromosome.
Quantification of the band intensities and back ground subtraction were conducted using Multi Gauge software (version 3.0; FujiFilm).
The pulsed EPR experiments were conducted using an X-band Bruker ELEXSYS E580 spectrometer equipped with an Oxford CF 935 cryostat.
Both were conducted using automated phone calls.
All analyses were conducted using MetaWin [30].
Analyses were conducted using Statistica 6.0 (StatSoft).
Most experiments were conducted using line 86.
BI analyses were conducted using MrBayes 3.1 [3.1.
All analyses were conducted using R [53].
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