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The relative percentages of mutant bands were calculated from gel images (TIFF format) using gel analysis tools in ImageJ (Schneider et al. 2012: http://rsbweb.nih.gov/ij/).
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The integral optical density of DNA bands was calculated from the area and the intensity of corresponding electrophoretic bands using Gel-Pro Analyzer 4.0 software (Media Cybernetics, USA).
31 The 2-SD band was calculated from the baseline data and graphed from the baseline phase through the intervention phase.
The mean dominant frequency (MDF), defined as the "center of mass" of a frequency band, was calculated from power spectra within delta (1 4 Hz), and theta alpha (6 10 Hz) bands, as the weighted mean of the frequencies included within the band, with their corresponding power values as weight.
Signal intensities from target protein bands were calculated relative to GAPDH signal intensities in liver homogenates.
Thus, we did not take these bands away from the total band numbers when the proportions of methylated bands were calculated.
Corresponding 90% confidence bands were calculated.
Band gap values were calculated from transmission measurements and ranged between 5.44 and 5.96 eV.
Different parameters such as crystallite size and band gap energy were calculated from the obtained data of XRD and UV vis, respectively.
To determine the expected number of events, the person-years at risk for the cancer cohort(s) summed within each calendar year, by age (5 year age bands) and by sex were calculated from the date of each cancer diagnosis to the date of death (or hospital admission) or to 5 years from the incidence date, whichever occurred first.
Time series of EVI2 (two-band enhanced vegetation index) were calculated from MODIS 500 m reflectance data and smoothed by a curve fitting procedure.
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