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The optical density of bands was quantitated using the ImageJ v.1.38 software and the ratios to GAPDH were normalized for time 0 hr and then expressed as fold- values of the mean±SEM.
The intensity of bands was quantitated using 'Phoretix 1D Advanced' software as above.
The integrated optical density of bands was quantitated using the Image J v.1.47 software.
The density of the respective bands was quantitated using a densitometer with AlphaView Software for FluorChem Systems (ProteinSimple™).
The density of bands was quantitated using ImageJ [ 28] and values were normalized to those obtained for sham animals.
The intensity of protein bands was quantitated using Lab Works 4.0 software (UVP Inc., Upland, CA, USA).
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Bands were quantitated using the integrated density function of ImageJ.
Bands were quantitated using National Institute of Health's ImageJ software.
Gels were vacuum dried, and Dvl1-3 protein bands were quantitated using a Typhoon phosphorimager with ImageQuant software (Molecular Dynamics).
Bands were quantitated using reverse image scanning densitometry (Photoshop CS2, Adobe) by normalizing the band intensity of the co-immunoprecipitated protein to the band intensity of the immunoprecipitated protein.
Radiolabeled bands were quantitated using a phosphorimager.
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