CGRMV was detected in tender shoots, leaves, bark and root tips, and the strongest bands were obtained using young leaves.
For quantification the optical densities of protein bands were obtained using ImageJ [ 18].
The blot was developed using a SuperEnhanced chemiluminescent detection kit, and then the membrane was placed in a Kodak Image Station 400 (Eastman Kodak Co., Rochester, NY, USA) for exposure and images of membrane signal bands were obtained using Kodak ID Software Eastman Kodak Co ... Real-time PCR was used to measure LC3 and Beclin-1 mRNA in H9c2 cells.
Finally, the membranes were exposed to X-ray films to detect the samples using an enhanced chemiluminescence development reagent (ECL system, Pierce, Thermo Fisher Scientific, Rockford, IL, USA) according to the manufacturer's instructions, and the photographs of the immunoreactive bands were obtained using a HP scanner (Andover, MA, USA).
A weak band was obtained using the GP5+/GP6+ assay following amplification from 225 ng DNA template but HPV was not detected at any of the other sample concentrations.
Intriguingly, no band shift was obtained using FRG1 Peak2 (Fig. 4B), further explaining the lack of activity of this region.
A similar band shift was obtained using the positive control TRIM48 (Fig. 4B), previously shown to be directly bound by DUX4 (44).
Additional evidence that the 58 kDa band resulted from phosphorylation was obtained using phos-tag™ SDS/PAGE immunoblots [ 39].
And the band gap of the NPs was obtained using a Perkin Elmer Spectrum Spotlight 300 FTIR microscope.
Information about the density distribution of the electronic states and the parameters of the energy bands of the ion-implanted GaAs films was obtained using Auger electron spectroscopy (AES), ultraviolet photoelectron emission spectroscopy (UPES) and high-energy electron diffraction (HEED).
