Immunoreactive bands were observed using an enhanced chemiluminiscence system (ECL; GE Healthcare, Barcelona, Spain).
This purified DNA was digested with restriction enzymes and the resulting bands were observed using agarose gel electrophoresis.
As the control, no protein bands were observed using the same antibody pre-absorbed with an excess of recombinant VDAC2 protein (Fig. 1B, lane 2).
The DNA bands were observed using an ultraviolet transilluminator.
The gel was exposed to a phosphor-screen, and bands were observed using Fuji FLA-3000 PhosphorImandr and quantified using MultiGauge 2.0.
Samples were incubated for 6-7 hrs at 37°C and the digested PCR products were resolved in 2percentt agarose gel electrophoresis stained with ethidium bromide and separated bands were observed using gel documentation system.
For quality and yield assessments, electrophoresis was performed for all DNA samples on 0.8% agarose gels that were stained with ethidium bromide; the bands were observed using a gel documentation system (Alpha Innotech, San Leandro, CA, USA) and compared with a known standard lambda DNA marker sample.
In contrast, with TRT-L20, TEL-1, TERT and Ab-2 antibodies, no specific stained band was observed using several cell line lysates or from tumour samples.
We observed in several cases that primers would generate multiple bands when using BAC DNA as the template, but only a single band was observed using genomic DNA as the PCR template.
As shown in Figure 3F, selective reduction of the p65 band was observed using anti-p65 antibody, whereas no reduction of DNA binding activity was observed with anti-p50 antibody.
