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The intensity of bands was determined using the Image J software package (open source).
Quantification of the protein bands was determined using densitometry scanning (BioRad 2000 Gel Doc system).
The intensity of bands was determined using Molecular Imaging apparatus (Vilber Lourmat, France) and BIO-1D software.
After development of the film, the image was scanned and the optical density of the bands was determined using the ImageJ software and Gel analyzer plug-in software.
The percentage of RNase H digestion (radioactive bands) was determined using a Storm PhosphorImager (Molecular Dynamics, Sunnyvale, CA) and the ImageQuant™ software for image analysis.
The relative intensity of the two bands was determined using the Molecular Imager System® Pharos FxPlus™ (Bio-Rad) and used to calculate the relative Pkd1 deletion (intensity KO band÷intensity WT band + KO band).
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The ratio of the average band intensities of the two immunoreactive bands of FRα (42 kd and 38 kd doublet) with the marker of individual subcellular fraction (FRα/ICAM-1, FRα/hsp90, FRα/vimentin, FRα/pRB, and FRα/H3 bands) were determined using densitometry (Fig. 1d).
After a baseline correction for the spectral range 1000 1800 cm−1, to remove fluorescence interference, the areas of the D and G bands were determined using a Lorentzian curve-fitting procedure by using Labspec v. 5.0 software (HORIBA Jobin Yvon Inc).
Optical density readings for the detected bands were determined using a computer-assisted densitometry program (ONE-Dscan 2.03, Scanalytics).
Intensities of all bands were determined using the Image-J 1.36b NIH software.
The intensities of the obtained bands were determined using Printgraph AE-6920-MF AE-6920-MF AE-6920-MF
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