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The volume fraction of the light-colored bands was defined as the area ratio of white pixels, and was regarded as the degree of development of the flow structure for the specimen.
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The uncertainty in the relative timing between ULF wave bands is defined as the summation of each individual ULF wave band uncertainty.
Type II regions correspond to bands that were observed in seven tissues but displayed different methylation patterns; these bands were defined as tissue-polymorphic methylation fragments.
Type I regions correspond to bands that were only observed in one tissue or bands that were found in six tissues but not in the seventh tissue; these bands were defined as tissue-specific methylation fragments.
Isolates that differed by 1 to 3 bands were defined as a subtype.
Isolates with identical patterns were considered genotypically "indistinguishable", while those that differed by 1 to 3 bands were defined as "closely related" and 4 to 6 bands as "possible related".
PFGE patterns that differed by <3 bands were defined as 1 PFGE type; isolates with the same PFGE patterns indicated indistinguishable strains, and those with 2 or 3 different bands indicated closely related strains (15 ).
After calculation of amplitudes of the spontaneous brain activity, frequency bands were defined as delta (1.3 4.0 Hz), alpha (8.0 12.0 Hz), low gamma (30.5 49.0 Hz), and high gamma (50.3 70.20 Hz).
Case-patients with isolates demonstrating indistinguishable genotypes, i.e., the same IS 6110-pattern designation for isolates with more than six IS 6110 bands or the same IS 6110 pattern and spoligotype for isolates with six or fewer IS 6110 bands, were defined as genotype clusters.
The high-frequency edge of the Raman band was defined as the minimum of the dI∕dv.
The high-frequency edge of the Raman band was defined as a minimum of dI∕dν, which is determined by curve fitting using a Gaussian function.
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