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Immunoblotting images presented here are representative of three experiments, and densitometric evaluation of the bands was conducted using UN-SCAN-IT gel densitizing software (Silk Scientific, Orem, UT).
Quantification of Western blot bands was conducted using Image J. Lymphoblastoid cell lines from 4 female controls and 4 female patients with high XIST expression were selected for examination of H3K27me3 on the inactive X chromosome.
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The ancestral character reconstructions (genome size, number of rDNA sites and number of CMA+ bands) were conducted using unordered maximum parsimony as implemented for continuous and meristic characters in Mesquite v. 3.02 software [ 103] using the 50%% majority-rule consensus tree resulting from the Bayesian inference analysis as the input tree file.
Images were scanned to a file, and band densitometry analysis was conducted using ImageJ (National Institutes of Health, Bethesda, MD).
For each subfrequency band, the ReHo analysis was conducted using DPARSFA.
Band integration of each spectrum was conducted using eFTIR software (Essential FTIR, http://www.essentialftir.com/index.html).html
As the numbers of CVI forms in each PCT were relatively small, standardisation was conducted using three age bands (0 15, 16 64 and 65 plus).
Analysis of PFGE profiles was conducted using the software Bio Image Whole Band Analyzer (Genomic Solutions, Ann Arbor, MI, USA).
Visualization was conducted using Bio-Rad ChemiDoc instrumentandon and software (Bio-Rad Labandtorintensityor band intensity was assessed using Image Lab 4.0 (Bio-Rad Laboratories), with correction for background and loading controls.
The study was conducted using lab mice.
Statistical analysis was conducted using SigmaStat 3.5.
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