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The average intensity of bands was calculated using ImageJ.
Quantification of bands was calculated using ImageJ (NIH), a Java-based image-processing program.
The intensity of the bands was calculated using Image J (http://imagej.nih.gov/ij/) on images taken using a G Box imager (Syngene, Cambridge, UK).
The amplified products were subjected to agarose gel electrophoresis, and the intensity of resultant bands was calculated using a Lane and Spot Analyzer (Atto, Japan).
The antibodies were considered specific based on previous data and detection of few bands in the blots, and in all cases the molecular weight of the corresponding bands was calculated using Quantity One software (BioRad).
Statistical significance of the mean IOD values for immunoblot bands was calculated using the two-way analysis of variance (two-way ANOVA) and Bonferroni multiple-comparison correction, when the comparison was made between the treatments (control and E2, P4, and E2 + P4) and duration of 3D culture (3, 6, 9, and 14 days).
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Eight of ten center frequencies of the local bands are calculated using Eq. (1) with k = 1 and (beta) = 1, 2, …, 8.
Films were scanned, and densitometric measurements of the bands were calculated using ImageJ.
The gray values of the bands were calculated using quantity one software (Bio-Rad, America).
The gray values of the bands were calculated using Bandscan 4.5 software (Glyko).
The intensities of the bands were calculated using ImageJ software (NIH), verifying non-saturation and subtracting the background.
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