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Following this a ratio of the intensities of the two bands was calculated and represented (as %) relative to the intensity of NP in the internal reference standard (PANC-1).
The size of DING proteins bands was calculated according to the migration of Precision plus protein kaleidoscope standard (BioRad) on the same gel for all experiments, except for the western blot on plasma samples, where Prestained Protein Ladder was used (Fermentas life science).
The intensity of bands was calculated with ImageQuant 5.2 software.
The average intensity of bands was calculated using ImageJ.
The gray value of bands was calculated by ImageJ 1.43 G (NIH, Bethesda, MD, USA).
Relative power in five frequency bands was calculated globally and for ten regions.
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The RMSE for non-superposed bands is calculated as follows.
The relationship for the various frequency bands are calculated for each year.
The energy bands are calculated at the current density of 50 A/cm2.
Back-of-the-envelope confidence bands are calculated as ε l,w ±1.96∗SE(ε l,w ).
The positions of the Si bands are calculated with a Schottky-Mott analysis.
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