A total of 158 bands were obtained and no polymorphic band pattern was observed.
A similar banding pattern was observed when endogenous IST1 was depleted and replaced by an siRNA-resistant wild-type IST1 construct (IST1R WT, lane 2 vs 4).
The banding pattern was observed under a phase-contrast microscope (400×) and interpreted with reference to the chromosomal map and nomenclature of Coluzzi and colleagues [ 9, 10].
When they were treated with HinfI, a 620 250 bps banding pattern was observed in all of the Anisakis larvae isolated from chum salmon, and a 380 300 250 bps banding pattern was observed in all of the Anisakis larvae isolated from chub mackerel.
The same banding pattern was observed using a well characterized MIF monoclonal antibody (III.D.9), suggesting that this banding pattern was not the result of antibody cross reactivity or non-specific binding (data not shown).
The intensity of bands corresponding to mutant was very strong as compared to the wild-type for the 0.1 pmole experiment, while for the 1.0 pmole experiment an opposite banding pattern was observed.
No differences in the banding pattern were observed between the wild type and mutant lines for the isoamylase-type DBEs, BEs, or amylolytic activities detected in this assay.
In the higher-energy region, a well-defined three-band pattern is observed with peaks at 364, 378, and 392 cm–1 that is unique to the M13V and M13V/K22M (see next paragraph) mutants, and not observed in wt.
Little similarity in banding patterns was observed among or even within HSGs (Additional file 3), suggesting divergence of genome structure.
With respect to the number of distinct banding patterns, a total of 81 banding patterns was observed in the sampled sites, ranging from 8 (PIR1) to 34 (PIR2).
