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Meanwhile, active Ras from mtDNA-KO was observed even when the quantity of LN ρ0-8 and MCF ρ0 lysates probed was reduced to 0.5 μg or 5 μg, respectively, active Ras was observed as two bands of intensity similar to the positive control.
Similar(59)
Inter-observer agreement as to the presence versus absence of a test band of intensity grade 2 or higher was 97.3%% (kappa 0.87), while agreement regarding the presence versus absence of a test band of intensity grade 1 or higher was 90.9 % (kappa 0.77), p < 0.001.
The amount of DNA in the cleaned-up product was quantified by comparing the intensity of the band to bands of known intensity in a HyperLadder marker (Bioline).
Its presence is also confirmed by a group of bands of weak intensity in 1490 1300 cm−1 region.
The profile generated by the multiple-primer P10 consisted of two bands of contrasting intensity(Figure 5).
Two bands of similar intensity were obtained.
These reactions generated PCR bands of similar intensity.
On Western blots, the ANGPTL4 protein showed bands of similar intensity throughout the six hours.
The assay includes a reference card with bands of graded intensity to facilitate the reading of results.
Sanger sequencing of 12 aN bands of sufficient intensity to sequence without further amplification, confirmed the events (data not shown).
Additionally, both pairs of enantiomers displayed two broad bands of lower intensity between 360 550 nm due to metal-based transitions.
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