Sentence examples for bands of expected sizes from inspiring English sources
PCR products were analyzed using agarose gel electrophoresis to identify bands of expected sizes.
Bands of expected sizes, as amplified from cDNA and not genomic DNA, were detected, and no bands were observed in the actin control when PCR reactions were programmed with RNA that was not subjected to reverse transcription (no RT).
Recombinant antigens were expressed as His6-tagged proteins in E. coli cells and appeared as single bands of expected sizes by SDS-PAGE, with molecular mass of 48 kDa for Eno and 39 kDa for Fba1.
Bands of expected size were observed neither in the untransformed nor uninduced bacterial extracts (Figure 4).
Bands of expected size were excised from the gel and dissolved in 4 volumes of 6 M NaI at 55°C for 5 minutes, followed by the addition of 15 μl hydrated silica suspension.
The amplified product was visualized as a single compact band of expected size under UV light and documented by gel documentation system (Biorad).
The products of the RT-PCR were analyzed on the agarose gel electrophoresis for a single band of expected size.
The criteria for specificity were that only a single band of expected size, and a single peak with expected melting temperature should be detected.
Full-length HA-tagged MAD2B (HA-MAD2B) proteins were obtained by transfecting U2OS cells, leading to the generation of a SDS-PAGE protein band of expected size, 28 29 kDa (Fig. 2A; Input).
Amplification of a band of expected size does not necessarily mean that the expected microsatellite motif is also present.
