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Two isolates were considered different if they showed 3 or more bands of difference.
Eight isolates harboring blaSPM-1 were typed, and by visual inspection all were considered closely related, presenting up to three bands of difference.
All isolates were not identical in PFGE profiles (50 pulsotypes were found), and only two isolates from southern Taiwan were closely related (within three bands of difference).
Other minor discrepancies were noted (designated as more than two bands of difference) for D6S29 (OAR6q17, BTA6q22) and C4B (OAR20q22, BTA23q12-q13).
Briefly, isolates with identical profiles were assigned to the same PFGE type; isolates with profiles differing by 1 to 6 bands were assigned to different subtypes within the same PFGE type; isolates with profiles showing more than 6 bands of difference were considered unrelated.
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In the case of the CA-MRSA IE reported here, the molecular typing revealed that although these strains share the same successful lineage ST5 with CA-MRSA (ST5-IV) and -HA-MRSA (ST5-I) epidemicloneses disseminated in this country, their PFGE pattern is closer to that of the CA-MRSA clone (one band of difference with I1, additional Figure 3).
In the case that the difference signal surpasses a defined threshold, the algorithm proposed computes the energy of the different frequency bands of the difference signal corresponding to one fundamental period, detecting a transient disturbance in the frequency domain within the next period of the input signal when a specific threshold is exceeded.
WT1 and D18 Hm fragments formed distinct bands of sufficient difference in size.
The carbohydrate bands of the difference spectra of the cells acquired in-line at the time points of sampling are in good agreement with the off-line spectra.
On a map, the regions look like undulating bands of color, with differences visible both north to south and west to east.
As a consequence, the amplitudes of the difference bands are much smaller than the absorption bands of the entire protein (difference bands may be smaller by a factor of 10−4, depending on the size of the protein).
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