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The presence of single bands in each sample was subsequently confirmed using DNA gel electrophoresis.
Proteins of different molecular weights were visualized as bands in each sample.
SDS-PAGE analysis of IS and AS protein-rich fractions revealed that the major bands in each sample were not the same (Fig. 3).
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Within the amount of protein loaded on the gel, we detected only a single band in each sample running with the molecular mass corresponding to TBC1D4-short and TBC1D4-long.
The corresponding functional groups (Ibarra et al. 1996; Maity and Mukherjee 2006) of the bands present in each sample are tabulated in Table 4.
Accordingly, the values of hyperphospho-BicD were normalized to the sum of both BicD bands in each individual sample.
It is found that there are three bands for each sample in the FT-IR spectra (Fig. 3).
The DGGE profiles from the experimental cultures showed few prominent bands in each of the samples and few similarities between profiles (Figure 6).
Except the band at 1470 cm−1, all ammonium bands in the sample spectrum are also present in the spectrum of aluminium ammonium sulfate dodecahydrate (ammonium alum, NH4Al SO4 2·12H2O) [32].
Telomere shortening was quantified by determining the percentage of telomere bands less than 1.4 kb to the total number of bands in the sample.
Several CSF-restricted IgGλ bands in this sample were clearly visible in all four runs.
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