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These bands could be competed out by an excess of unlabeled (cold) NFκB probe.
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Supershift assays confirmed that the lower band could be competed by excess cold, but not by mutant, probe while the upper band could be displaced by incubation of the nuclear extract with an antibody against the p50 or the p65 complex (Figure 4).
The intensity of one of the bands was increased when cells were cultured at 32°C compared with those cultured at 37°C, and could be competed out by a 50-fold molar excess of the unlabeled wild-type, but not mutant, probe indicating a specific interaction.
Now it could be competing directly with its partners.
Another possible explanation could be competing mortality.
These bands could be induced in presence of toluene.
Multiple bands could be detected on native-PAGE zymogrm.
The result confirmed that the non-canonical E-box motif CACGCG (−697 to −692) could bind to the nuclear extracts, the specific binding band lessened significantly because the probe was methylated, while normal labeled probes could not be competed off by the unlabeled methylated wild-type probe.
The band could be poignant, too.
A second band could be detected at 110 kDa.
Primer pairs for which a faint band could be seen.
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