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Following slide preparation, various staining techniques such as classic staining (e.g., aceto-orcein, haematoxylin, Giemsa, Wright and Leishman stains) or banding techniques (e.g., Q-banding, G-banding, R-banding, C-banding, and high-resolution banding) (Calado et al. 2013; Moore and Best 2001; Wang et al. 2010) are used to stain chromosomes for different purposes.
Identification of individual chromosomes based on size and banding patterns is difficult in Lepidoptera because of the large number of small and equally sized chromosomes that are not susceptible to banding techniques during mitosis.
Recent cytological improvements with various banding techniques enabled a further study on the origin of nondisjunction.
4, 5 The development of banding techniques and high-resolution chromosome analysis enabled the detection of subtle rearrangements that affected one or a few chromosome bands.
Most of the cancer-causing translocations were detected by chromosome banding techniques [ 2], and they provided rewarding entry points for the positional cloning of oncogenes.
Metaphase cells were prepared using standard cytogenetic techniques, and air-dried slides were prepared and stained using Trypsin Giemsa banding techniques as described previously (35).
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Examples include karyotyping by G-banding techniques based on morphological similarity (Drets and Shaw 1971); fluorescence in situ hybridization (FISH) (Bauman et al. 1980), such as M-FISH based on DNA sequence homology (Speicher et al. 1996); and array-based methods, such as array comparative genomic hybridization (array-CGH) (Solinas-Toldo et al. 1997).
The study reports detailed chromosome morphometric analyses with fluorescence banding technique, differential distribution of heterochromatin, flow cytometric nuclear DNA contents and meiotic behavior of the native populations of Benincasa hispida, Luffa cylindrica and Trichosanthes dioica in India.
The cytogenetic studies were performed using Trypsin Giemsa banding technique.
The chromosome analysis was performed using a trypsin-Giemsa banding technique.
The chromosome slides were prepared from short-term-cultured tumor cells using the standard trypsin Giemsa banding technique.
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