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One sample from each banding patterns were sequenced.
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Six most common genetic variants with distinct electrophoretic band patterns marked with capital letters from A to F are presented in Figure 2. To evaluate the differences in the nucleotide sequence between the individual genetic variants, all clones showing distinct band patterns were sequenced by the Sanger method.
Samples with an aberrant band on the SSCP gel or an altered DHPLC pattern were sequenced by reamplifying the corresponding exon.
Samples that exhibited aberrant bands on the SSCP gel or an altered DHPLC pattern were sequenced by reamplifying the corresponding exon.
Once the SSCP-HD banding patterns were reconstituted successfully, the cDNA sequences were subjected to additional primer design for isolation of the corresponding genomic sequences spanning exon 1 to exon 3. SSCP and HD are two mutation-scanning techniques that may be run on the same polyacrylamide gel [26], and therefore do not require separate experiments.
Given this level of resolution, different banding patterns were discovered that belonged to the same prey OTU by sequence analysis.
Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
The PCR banding patterns were scored 1 5.
The amplified banding patterns were scored as 'A' for ICCV-2 type banding pattern, 'B' for JG-62 type banding pattern and 'H' for heterozygous loci.
The chromosome number and banding patterns were analyzed with a 300 500 band resolution.
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