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The PCR banding patterns were scored 1 5.
The amplified banding patterns were scored as 'A' for ICCV-2 type banding pattern, 'B' for JG-62 type banding pattern and 'H' for heterozygous loci.
This PCR reaction was carried out for four independent single-worm genomic DNA samples from each of the 22 global-clade C. briggsae natural isolates analyzed using agarose gel electrophoresis; gel banding patterns were scored to evaluate heteroplasmy patterns in their mitochondrial genomes.
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A template is supplied by the manufacturer to help read the strips Appendix 1 where the banding patterns are scored by eye, transcribed and manually fed into the Laboratory Information System LISS).
Band patterns were scored in AFLP and ISSR fingerprint experiments and binary matrices for analysis were constructed for all populations of each species (POPGENE Version 1.32 [ 57]).
Band patterns were scored as presence/absence dominant markers with aid of the Quantar Pro band analyser software (Keygene, Wageningen, Netherlands), and independent replicates were performed for several TRAP-marker combinations in order to verify and validate band-scoring results.
The amplicon band
Primer combinations were excluded from the study if their banding patterns were difficult to score or if they failed to amplify consistently in all lines.
All 305 samples of group II were tested negative and all of 205 positive samples (group III) showed a clearly visible LPS banding pattern and were scored positive.
One sample from each banding patterns were sequenced.
Banding patterns were analyzed visually.
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