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Once the SSCP-HD banding patterns were reconstituted successfully, the cDNA sequences were subjected to additional primer design for isolation of the corresponding genomic sequences spanning exon 1 to exon 3. SSCP and HD are two mutation-scanning techniques that may be run on the same polyacrylamide gel [26], and therefore do not require separate experiments.
Similar(59)
From the reactive atom as starting point, an atomic pattern is reconstituted within a distinct distance.
One sample from each banding patterns were sequenced.
Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
The PCR banding patterns were scored 1 5.
The amplified banding patterns were scored as 'A' for ICCV-2 type banding pattern, 'B' for JG-62 type banding pattern and 'H' for heterozygous loci.
These banding patterns were (re)analysed by one person (JCH).
Banding patterns were compared with 3.0% optimization and 2.5% band position tolerance.
The chromosome number and banding patterns were analyzed with a 300 500 band resolution.
After ethidium bromide staining, banding patterns were compared visually.
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