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Restriction-fragment banding patterns were matched digitally using a Gel doc 1000 System (Bio-Rad, Hercules, CA) and compared using the Molecular Analyst Plus software (Bio-Rad).
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Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
The PCR banding patterns were scored 1 5.
The amplified banding patterns were scored as 'A' for ICCV-2 type banding pattern, 'B' for JG-62 type banding pattern and 'H' for heterozygous loci.
The chromosome number and banding patterns were analyzed with a 300 500 band resolution.
Banding patterns were compared with 3.0% optimization and 2.5% band position tolerance.
These banding patterns were (re)analysed by one person (JCH).
Banding patterns were analyzed with BioNumerics software, version 5.0 (Belgium).
After ethidium bromide staining, banding patterns were compared visually.
Two distinct types of banding patterns were observed.
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