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Amplified Fragment Length Polymorphism (AFLP) banding patterns were generated using a modified version of the protocol described in [30], that allowed for fluorescent detection of labeled AFLP's.
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After imprinting, distinctive color patterns are generated.
DNA banding patterns were analyzed using bioNumerics software (Applied Maths) to calculate Dice coefficients of correlation and to generate a dendrogram using the unweighted pair group method of arithmetic averages (UPGMA) clustering.
One sample from each banding patterns were sequenced.
The resulting RFLP banding patterns were compared visually.
Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
The PCR banding patterns were scored 1 5.
The amplified banding patterns were scored as 'A' for ICCV-2 type banding pattern, 'B' for JG-62 type banding pattern and 'H' for heterozygous loci.
The chromosome number and banding patterns were analyzed with a 300 500 band resolution.
These banding patterns were (re)analysed by one person (JCH).
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