Band patterns were evaluated using the Dice similarity coefficient with a 4% band tolerance and 1% of optimization.
Band pattern was evaluated and all analyzed plants were verified as hybrids, including the intraspecific cross between K. blossfeldiana cultivars.
PFGE patterns were evaluated visually, and isolates were assigned to the same pulsotype when exhibiting a difference of <3 bands from the index isolate.
Ecological aspects such as abundance and diversity patterns are evaluated.
This PCR reaction was carried out for four independent single-worm genomic DNA samples from each of the 22 global-clade C. briggsae natural isolates analyzed using agarose gel electrophoresis; gel banding patterns were scored to evaluate heteroplasmy patterns in their mitochondrial genomes.
Additionally, each pattern is evaluated separately.
One sample from each banding patterns were sequenced.
The resulting RFLP banding patterns were compared visually.
Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
