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Banding patterns were detected by HRP-conjugated goanti-rabbit-antibodyory or anti-mouse (Calbiochem) developed for enhanced chemiluminescence (Amersham/GE Healthcare) according to the manufacturer's instructions, with exposure to Kodak Xray films for 1, 5, 10, 20 min and over night.
Multiple banding patterns were detected at three enzyme loci and one enzyme had the potential to be a genetic marker between two geographically separate populations.
Similar(57)
Alteration in the AFLP banding patterns was detected in both calli and regenerants of all six genotypes compared with their respective seed-plants as controls.
For instance, at the dimeric enzymes ENOL, GAPD, GOT and TPI individual worms showed single banded homozygous and the typical three banded heterozygous patterns, whereas at the monomeric enzyme, PGM, typical double banded heterozygous and single banded homozygous patterns were detected.
No geographical or host patterns were detected.
Six main patterns were detected.
No seasonal patterns were detected.
A biphasic pattern was detected.
Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
The PCR banding patterns were scored 1 5.
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