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The amplified products were electrophoresed on 4% polyacrylamide gels, and the banding pattern was visualized using a non-radioactive silver staining method, as described by Panaud et al. (1996).
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The DNA band pattern was visualized under UV light using ethidium bromide staining.
DNA banding patterns were visualized with 0.1% ethidium bromide and digitally photographed.
DNA banding patterns were visualized under UV light with ethidium bromide staining.
Banding patterns were visualized by ethidium bromide staining and photodocumented under UV light.
The amplified products were separated on a 1% agarose gel and banding patterns were visualized with ethidium bromide staining and UV illumination with a gel documentation system (Gel Doc 2000; Bio-Rad, UK). S. pneumoniae isolates were streaked on blood agar and incubated at 37°C for 18 hours.
The obtained band patterns were visualized by mean of gel electrophoresis and analysed by using the free software gelAnalyser (http://www.gelanalyzer.com).
After PFGE, the band patterns were visualized by ethidium bromide staining and then photographed (GelDoc™ imaging system, Bio-Rad, Munich).
Band patterns were visualized with a silver staining method [ 54].
After gel electrophoresis, band patterns were visualized with silver staining, and gel images were taken with a digital camera.
After reaction, MSSCP analysis was performed and ssDNA band patterns were visualized as presented in Figures 3 and 4. The presence of all Australian genetic variants was clearly detected in combinations with each other and with vaccine Roakin strain.
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