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The localization of the signals relative to the DAPI banding pattern was resolved by merging digital images using Adobe Photoshop.
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A unique banding pattern was defined as a pulsotype (PT).
The banding pattern was consistent with L. infantum.
In 2-D gel this BN gel band was resolved into five proteins (spot 1-5).
OPA1 band pattern was analysed by standard western blot procedure.
The most complex band pattern was found in M. ramiflorus.
This three band pattern was designated the 'bird-type' RFLP pattern.
5.0 μg of each of DNA samples were resolved by electrophoresis for 4 h on a 1.5% agarose gel and stained with ethidium bromide and the banding patterns were visualized with the Foto/Eclipse UV transilluminator.
One sample from each banding patterns were sequenced.
Banding patterns were analyzed visually.
Identical banding patterns were called pulsotypes.
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