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Karyotype analysis was performed using peripheral blood lymphocyte cultures and a standard GTG banding method [ 8].
Metaphase spreads were stained by standard trypsin-Giemsa banding method and analyzed under microscope as described previously [ 7].
For karyotype studies of the xenograft, the Giemsa G banding method was performed after 6 and 12 passages.
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These methods are labeled as narrow banding methods.
By classical banding methods, 15/18 patients showed at least one aberration characteristic for primary glioblastoma (+7 in 7/18, deletions of 9p in 10/18 and −10 or deletions from 10q in 8/18 patients).
Classical banding methods provide only basic information regarding the structure and identities of chromosomes, while spectral karyotyping has the potential to provide improved characterization of aberrant chromosomes that contain DNA sequences [3].
Interpretation of the complex genomes characteristic of solid tumours is also technically difficult using conventional cytogenetic banding methods.
Note that the multiple principal band method requires more computation than the spectral eigenface method.
Nudged elastic band method was also utilized to probe the ions diffusions.
In contrast to the G band position method, the 2D band method depends not only on band position but also on band shape.
A maximum entropy principle is developed in the general framework of the band method.
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