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Banding intensity was used to identify heterozygotes.
Based on visual comparison of banding intensity, there is higher rDNA expression of T. dubius-origin units than of T. porrifolius-origin units in all plants from all populations examined, except for individuals from the Palouse population (Additional file 3).
Briefly, a positive WB result indicates reactivity to at least p24 and 1 of the 3 env (gp41, gp120, or gp160) proteins with banding intensity at least as strong as that seen in the weak-positive control; a negative WB result indicates no reactivity and an indeterminate WB result indicates reactivity to at least 1 protein.
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b Purified protein sample band intensity.
The R1 band intensity changes non-monotonously.
Subtraction then gives a band intensity value.
Band intensity was measured by densitometry.
Band intensity was determined by phosphoimager quantification.
Band intensity was measured using ImageJ software.
Band intensity was quantified by densitometry.
Band intensity was normalized to the input band.
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