Sentence examples for band when digested with from inspiring English sources

Southern blot analysis using the Hpt probe identified three hybridization bands when the genomic DNA was digested with HindIII, but only one band when digested with XbaI (Fig.  4e, lanes 6 to 9).

LNT01 (recipient), wild type and plasmid-tagged donor strains, and three clones of each transconjugant all exhibited unique restriction banding patterns when digested with SmaI, XhoI or NarI.

Hybridization with probes created from cDNA fragments of HlMyb2, HlbHLH2 and HlWDR1 genes showed single bands for all tested genes when digested with EcoRI (HlbHLH2 and HlWDR1), XhoI (HlbHLH2 and HlMyb2), XbaI (HlMyb2 and HlWDR1), PstI (HlbHLH2), BglII (HlMyb2) and BamHI (HlWDR1) enzymes.

The DdeI restriction site is lost in jp so amplified DNA, when digested with DdeI, will yield one band at 125 bp for jp males, two bands at 75 and 50 bp for wild-type males and females, and all three bands for heterozygous females.

In the case of the N346H mutation in the regulatory subunit of AHAS from A. thaliana [ 11], the second PCR product obtained using 0.05, 0.1 and 1.0 pmole of the first flanking primer, when digested with KpnI, showed different relative band intensity patterns when run on 1% agarose gel.

When digested with methylation-insensitive restriction endonucleases, gDNA was fully digested; we observed no banding pattern differences at any time point in TDCPP-treated embryos compared with vehicle controls.

The band was digested with trypsin and subjected to tandem MS-MS analysis.

The DNA of the band was digested with Sau 3A and then size fractionated on an agarose gel.

These bands were digested with trypsin overnight at 37 °C.

Tau bands were digested with Lys-C and phosphopeptides isolated and identified as described in Methods.

When digesting casein with a protease like Alcalase, the peptide formation occured within a few minutes and most casein protein components were digested after 30 60 min (Fig. 3a).