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The activity band was visualized with UV light as a bright band against a dark background.
The activity band was visualized with UV light as described above.
After a 1-h antibody incubation and three washes with PBS-1X buffer, the δ-SG protein band was visualized with a chemiluminescence reagent (Supersignal, WestPico, Pierce) and exposed to X-ray film.
After 45 min of antibody incubation and five washes with TTBS 1× buffer, the protein band was visualized with a chemiluminescence reagent (Supersignal, WestPico, Pierce) and exposed to X-ray film.
By immunoblotting we were able to detect the p18 signal using polyclonal affinity purified antibodies generated with peptides CSD1-3, but no band was visualized with the YB-1 N-term, C-term or DbpA N-term antibodies.
After transfer to the hybridization transfer membrane (PerkinElmer, Boston, MA) and hybridization with a PCR-generated probe derived from primers targeting blaOXA-72 (Additional File 2), the band was visualized with a digoxigenin (DIG) DNA labeling and detection kit (Roche Diagnostics, Basel, Switzerland) according to the manufacturer's instructions.
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The nucleic acid bands were visualized with ethidium bromide staining.
Bands were visualized with Western blotting Luminol Reagent (Santa Cruz).
Electrophoretically separated protein bands were visualized with the Colloidal Blue staining kit (Invitrogen).
Protein bands were visualized with using enhanced chemiluminescence detection reagent (ECL).
The bands were visualized with enhanced chemiluminescence (GE Healthcare) on a LAS3000 machine (FUJIFILM).
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