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Afterwards protein band was visualized according to Blum et al. with slight modifications, excised, washed and in-gel digested as described previously.
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β-actin immunoreactive bands were visualized according the procedure described above.
Bands on blots were visualized according to Li-Cor Odyssey standard protocols.
The aromatic amino acid: 2- oksoglutarate activity bands after native PAGE were visualized according to the method described by Pedraza et al. (2004) with modifications.
Protein band was visualized using ECL and Kodak film.
Protein bands were visualized using ECL GE Healthcaree) according to manufacturer's protocol and Amersham Hyperfilm ECL (GE Healthcare).
The immunoreactive bands were visualized by chemiluminescence according to the manufacturer's recommendations (Thermo Fisher, Waltham, MA, USA).
The cDNA bands were visualized by silver staining according to Bassam et al. [ 23].
After being washed with TBST, the blots were incubated with an HRP-conjugated anti-IgG and the target bands were visualized with ECL plus reagents according to the manufacturer's instructions.
Immunoreactive bands were visualized with ECL Plus (Amersham), according to the manufacturer's instructions.
Subsequently, the membrane was incubated with appropriate secondary antibody, and the immunoreactive bands were visualized using enhanced chemiluminescence kit according to the manufacturer's instructions.
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