The DNA bands were extracted using a gel extraction kit (QIAquick, QIAGEN Sciences, Germantown, MD, USA) and sequenced directly using a BigDye Terminator Cycle-sequencing kit (ABI PRISM 310NT Genomic Analyzer, Perkin-Elmer Applied Biosystems, Foster, CA, USA).
All visible bands were extracted using Qiagen QIAquick gel extraction kit (Crawley, UK) and sequenced.
Bands were extracted using a razor blade and stored at -20°C until gel extraction was performed.
Identified gel bands were extracted using the PureLink Quick Gel Extraction Kit (Invitrogen) and Sanger sequenced to identify excision products.
Bands were extracted using a QIAquick Gel Extraction Kit microcentrifuge protocol (Qiagen Sciences, Valencia, CA) and sequenced on an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA) using Big Dye Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, Foster City, CA).
The vector and insert bands were excised and DNA was extracted using Qiaquick gel extraction kit (QIAGEN, Valencia, CA, USA).
The emission of the PCP complexes was extracted using a band-pass filter, HQ675-20.
Bands of interest were excised and the DNA was extracted using the QIAquick® Gel Extraction Kit (Qiagen).
Total RNA was extracted using TRIzol method.
RNA was extracted using RNeasy columns (Qiagen).
