Sentence examples for band was excised using from inspiring English sources

Exact(2)

The gel was then reassembled and the identified size selected band was excised using a clean scalpel blade.

The gel was then visualised on a Dark Reader Transilluminator and a band of gel corresponding to the 250 bp ladder band was excised using a GeneCatcher Disposable Gel Excision tip.

Similar(58)

The PCR products were electrophoresed in 1.0% agarose gels, bands were excised using a razor blade and purified using the BioTeq PCR Quick Gel Extraction Kit (BioTeq Inc).

The PCR product segments were electrophoresed in 1.0% agarose gels, bands were excised using a razor blade and purified using the BioTeq PCR quick Gel Extraction Kit (BioTeq, Inc, USA).

Bands of interest were excised using sterile scalpel blades from multiple replicate (2 10) samples, which were subsequently sequenced, in order to ensure that bands of the same mobility shared the same sequence identity.

The bands of interests were excised using a Wizard SV Gel and PCR Clean-Up system (Promega, Fitchburg, WI, USA), then sequenced directly or cloned into a vector using a TOPO-TA Cloning Kit (Invitrogen) with standard cloning techniques.

The mononucleosomal DNA band was excised and purified using the QIAquick Gel Extraction Kit (QIAGEN Inc).

The gel was stained with ethidium bromide and the desired DNA band was excised and eluted using QIAquick Gel Kit (QIAGEN).

Ten microlitres of each PCR product was electrophoresed through a 1% agarose gel to determine amplicon size, and the gel band was excised for purification using a GFX DNA Purification Kit (Amersham Pharmacia Biotech Inc).

Proteins in the IGs and IDVs were extracted by using SDS sample buffer, resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Figure 1C), and each visible protein band was excised for protein identification using MALDI-TOF mass spectrometry.

The expected band was excised from gel and purified using Midi gel extraction column (Advanced Microdevices Pvt. Ltd .. Using TA cloning vector, insert was cloned in pGEM-T easy vector.

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