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The relative optical density (R. O. D). of the band was estimated using NIH ImageJ 1.36b software.
The amount of radioactivity in each band was estimated using a Fuji phosphorimager.
The integral optical density (IOD) of the Western blot band was estimated using ImageJ.
Coomassie-stained protein bands were ultimately scanned, and the intensity of each recombinant protein band was estimated using densitometry (Duoscan T1200, Bio-Rad, Hercules, CA).
Gel images were captured using the image acquisition software Gene Snap (Syngene Ltd., UK) on a Gene Genius Bio Imaging System (Syngene Ltd., UK) and the concentration of DNA in each amplified band was estimated using the GeneTools (Syngene Ltd., UK) analysis programme against the known molecular weight standards included on the gels.
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Molecular mass of the protein bands was estimated using the 198 6.8 kDa molecular mass protein standards (Bio-Rad Laboratories, California, USA).
Exposed films were scanned and the density of equal areas of the developed bands was estimated using Un-Scan-It software (Silk Scientific).
The optical density (O.D). of the bands was estimated using an HP scanjet 3670 scanner (HP, Madrid, Spain) and the Scion Image Alpha 4.0.3.2 free analysis software (Scion Corp., Frederick, MD, USA).
The bands of amplicons were then imaged under UV light using the Flo Gel FGIS-3 fluorescent gel image system (Top BIO Co., Taipei, Taiwan), and the sizes of bands were estimated using Quantity One software version 4.62 (Bio-Rad Laboratories, HerCaliforniaifornia, USA).
The product band intensity was estimated using Image J software (http://rsbweb.nih.gov/ij/) and normalised using GAPDH.
The immunoreactive band intensity was estimated using a computer-assisted gel scanning densitometer (GS-710 Callibrated Imaging Densitometer) and Quantity One software, both from Bio-Rad (Hercules, CA, USA).
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