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The integrated optical density of each band was determined using GelPro® software (Media Cybernetics, Bethesda, MD).
To quantify the WB signal, the integrated optical density (I.O.D) of a given band was determined using Scion Image software (Scion Corporation, Frederick, MA).
The volume densitometry of each band was determined using ImageMaster 2D software.
The thickness of the fibrous collagenous band was determined using ImageJ and presented in Figure 5.
The intensity of each band was determined using Image J software (nih.gov).
Western blots were scanned and the integrated intensity of each band was determined using Image J Schneiderr et al., 2012).
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Orientation of the crack band is determined using the maximum principal stress.
The location of hypothalamic structures and the diagonal band were determined using human atlas coordinates [37].
The optical density values of each target protein band were determined using NIH Imaging software.
The intensity of bands was determined using the Image J software package (open source).
Quantification of the protein bands was determined using densitometry scanning (BioRad 2000 Gel Doc system).
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