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The LISS III images were corrected for atmospheric path radiance using dark object subtraction method (Chavez 1988), wherein the pixel (associated with the dark object) having minimum brightness value in the near infrared band was detected and the corresponding pixel values in all other bands were subtracted from the specific raw bands.

In AhR/ARNT transfected αTN4 cells, a much stronger band was detected and TCDD addition further increased the band intensity (Fig. 9B, C).

In six of the seven cases a single predominant hybridizing band was detected and the observed size matched the predicted size within the limits of resolution of Northern blotting [see Additional file 2: Figure S4].

Phoretix 1D Advanced software was also used to determine the volume of each band (with band volume the product of the area over which a band was detected and the intensity of signal recorded over that area).

A sample was considered to be positive for CDKN2A methylation when a methylated band was seen in both stage 2 PCR replicates using the methylated primers; negative for CDKN2A methylation when only an unmethylated band was detected; and non-evaluable when no band was seen using either the methylated or the unmethylated primers.

The intensity of the specific bands was detected and analyzed by Odyssey infrared imagine system (Li-Cor).

The bands were detected and revealed by applying enhanced chemiluminescence (ECL) using ECL western blotting detection reagents and exposed to X-ray film (GE Healthcare, Little Chalfont, Buckinghamshire, UK).

Depending on the electrode potential different infrared bands were detected and assigned.

From 29 reliable primers that were used, 369 bands were detected and from which 314 (85.44%) bands were polymorphic.

After excitation, two overlapping bands were detected and identified as the S1 state and the first triplet state T1.

All of the active sub-bands were detected and all three users identified.