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Relative-intensity band quantifications were determined using ImageJ (1.40 g; http://rsb.info.nih.gov/ij/).
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Band density trace and quantification were determined using ImageJ National Institutes of Healthh).
Protein quantification was determined by BCA assay.
Absolute quantification was determined using a genomic DNA standard curve.
Relative quantification was determined.
For quantification, membranes were scanned and band intensities were determined with the image analysis software ImageJ (http://rsb.info.nih.gov/ij).
Band intensities were determined by scanning the exposed films followed by quantification using the Image Gauge 3.0 software (Fujifilm, Düsseldorf, Germany).
For quantification, the densities of bands were determined using Gene Tools software (Syngene), and normalization to the corresponding loading control.
SDS−PAGE and band quantification were performed as described for pulse proteolysis.
Quantification of band density was determined using Quantity One software (Bio-Rad, version 4.4.0) and reported in optical density per square millimeter.
Band quantification was performed using the ImageQuant 5.0 software package (Molecular Dynamics) and the amount of protein required to shift 50% of the probe was determined using SigmaPlot.
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