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Band patterns were scored in AFLP and ISSR fingerprint experiments and binary matrices for analysis were constructed for all populations of each species (POPGENE Version 1.32 [ 57]).
The amplicon band patterns were scored according CP codes (eg. <abxcd>, <efxeg>, <lmxll>, <nnxnp> and <hkxhk>) and missing data was replaced by "--" as described by Ooijen and Voorrips (2001) [ 39].
Band patterns were scored as presence/absence dominant markers with aid of the Quantar Pro band analyser software (Keygene, Wageningen, Netherlands), and independent replicates were performed for several TRAP-marker combinations in order to verify and validate band-scoring results.
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The PCR banding patterns were scored 1 5.
The amplified banding patterns were scored as 'A' for ICCV-2 type banding pattern, 'B' for JG-62 type banding pattern and 'H' for heterozygous loci.
This PCR reaction was carried out for four independent single-worm genomic DNA samples from each of the 22 global-clade C. briggsae natural isolates analyzed using agarose gel electrophoresis; gel banding patterns were scored to evaluate heteroplasmy patterns in their mitochondrial genomes.
A template is supplied by the manufacturer to help read the strips Appendix 1 where the banding patterns are scored by eye, transcribed and manually fed into the Laboratory Information System LISS).
The band patterns were compared visually.
Band patterns were analyzed to determine clonal identity.
Band patterns were compared using Gelcompar II (Applied Maths, Belgium).
Band patterns were visualized with a silver staining method [ 54].
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