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BAC clones that produced similar band patterns were considered to overlap and were grouped into contigs.
Similar(59)
Loci resulting in polymorphic banding patterns were considered suitable for further analysis.
Post treatment and primary infection parasites showing identical bands were considered as recrudescence, whereas non-identity in banding patterns was considered as newly acquired infections.
Isolates showing an identical banding pattern were considered indistinguishable, and those showing differences of 3 bands were considered closely related.
PFGE patterns were considered identical if they shared every band, similar if they differed from one to three bands, and distinct if they differed by four or more bands (12 ).
Patterns were considered different when the profiles differed by at least 1 band.
The fingerprints were compared visually, and patterns were considered different when they differed by at least one amplification band.
Isolates with indistinguishable patterns were considered identical PFGE types while those with at least one band difference were considered to be different types.
Isolates with identical patterns were considered genotypically "indistinguishable", while those that differed by 1 to 3 bands were defined as "closely related" and 4 to 6 bands as "possible related".
RFLP patterns were considered to match if the patterns were identical or differed by the addition or subtraction of a single band.
Only bands with a present-absent pattern were considered.
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