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As described previously, pretreatment of cells with MG132 also modulated the band pattern of these nuclear fragments (Figure 2D).
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The band pattern of the floating layer showed no differences to the associated digestate (Figure 9c).
The PFGE band pattern of the isolate from patient 2 and the pattern from the patient's household (lanes 10 and 11) appear almost identical ("closely related").
Positive clones were screened on a DGGE gel to determine their electrophoretic mobility compared with the band pattern of the original environmental sample.
Finally, the SC-PS152 isolate, whose genotype is widely associated with Nor98 cases (33, 34 ), showed the characteristic band pattern of the atypical (Nor98-like) scrapies (4, 35 ).
At the same concentration of wild-type protein, we observed that mutants R18A, I141A, L143A, E153A, C376A/G377A, Y393A, I432A, V566A and V571A had an approximately 2 7 fold decrease in DNA binding affinity as compared with wild-type protein (P<0.05), and the band patterns of these mutants were different (Fig. 5 B).
No differences were observed in the glutenin band patterns of the wheat and whole wheat flours and their tarhana samples.
As shown in Figure 3C, the phosphorylation band patterns of the endogenous Mis17-FLAG remained nearly constant after 4.25 h.
SDS-PAGE of the obtained fractions indicates that separation into subfractions was successful as the protein band patterns of the fractions differ considerably (Figure 1a).
The dendrogram was constructed from the results of the band patterns of the PFGE analysis.
The band patterns of the DGGE gel were analysed using Image J software (available for free download at http://rsb.info.nih.gov/ij/).
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