Sentence examples for band of correct size from inspiring English sources
Indeed, as shown in Figure 2B (right lower panel), a single dominant PCR band of correct size was observed in the recombinant clones, but not in the parental TrxR1-YFP cells.
A sample from the serial dilution was separated on an ethidium bromide stained agarose gel, to confirm that a single band of correct size was amplified.
All amplified products were run on a 1.5% agarose gel, and the gel band of correct size was extracted and purified using Qiagen's Qiaquick Gel Extraction Kit as per the manufacturer's instructions.
Inoculate 2 colonies that show a single band of correct size in LB + Carbenicillin (100 μg/L) or Ampicillin (100 μg/L), and incubate over night for plasmid isolation.
RT-PCR conducted with these primers produced bands of correct size and sequence with RNA from the DUGV-like virus isolates, while RT-PCR results using these primers with RNA from DUGV and BHAV were negative.
For SLC1A3, no protein band of the expected size was detectable for either of the reported splice variants (~60 and ~55 kDa) [ 27], using 3 different antibodies which all gave clear bands of correct size in positive control mouse brain tissue (not shown).
Upon confirming amplification of correct size band the PCR product was purified with PCR purification kit (Qiagen, Valencia, CA) and sequenced using the same forward and reverse primers.
The activation signal upon ligand binding was only present in cells over-expressing IR-B showing a band of the correct size of the β-subunit of the insulin receptor (~95 kDa).
As others have commented (Mogensen et al, 2002), a typical immunoblot with APC N15) of whole-cell lysate reveals a number of intense bands between 40 and 90 kDa (one of these intense bands is Ku80), but only a weak band of the correct size for APC itself.
In cases where multiple bands were observed in the PCR product, the band of the correct size was excised and purified using the Wizard® Gel and PCR Clean-Up kit (Promega) before being used as a template for IVT.
