Sentence examples for band isolated from inspiring English sources

Products of ∼200 ± 25 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel, purified using Gel Extraction kit (Qiagen, Hercules, CA), and PCR amplified using Phusion Polymerase with Illumina primers for 18 cycles.

DNA was separated by agarose gel electrophoresis, one band isolated and the agarose fragments containing the DNA divided in half.

However, the sequence of the 100-bp band isolated from this sample from Sardinia (Italy) did not match any of the five sequences of P. mauritianus previously isolated (Lambertini et al. 2012).

Mass spectrometry analysis of the H3 protein band isolated from mononucleosomes after HMTase assays independently confirmed H3-K27 as the site being methylated, and it revealed that Pcl-PRC2 and rPRC2 both trimethylate H3-K27.

Of the 98 kDa band isolated from total C4-2 celysatestes (by columns of HGF-coated agarose beads), a total of 24 peptides were sequenced, corresponding to approximately 30% of the protein sequence of nucleolin, with 100% identity match to the previously published protein sequence (NCBI 4885511; Table 2).

The level of the latter band was equal to that of the band isolated from neuronal cultures treated with a highly selective BACE1 inhibitor compound 3 (Fig 3A), which is in accordance with our hypothesis that the E682K mutation blocked C89 generation.

ChIP DNA and input DNA were first band-isolated on a 2% agarose to obtain fragments between 150 and 350 base pairs and DNA was extracted using the QIAquick gel extraction kit (Qiagen) and eluted in 34 μl.

The band isolates its symmetry and amplifies it — several bright white kliegs illuminate the band and do not move or vary colors during the course of their set.

An optimal algorithm for evaluating zero band isolates is yet to be determined and should be based on evolving secondary methods.

Results from Illumina sequencing were compared to those from dideoxy chain termination sequencing of bands isolated from following denaturing gradient gel electrophoresis (DGGE) analysis of amplified PCR products; a method traditionally used for the separation of bacterial species.

Of these 61 patients, 20 received treatment only for platysma bands (isolated closed platysmotomy).