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The intensity of the band in each lane was normalized to the intensity of β-Actin.
The gels corresponding to the different ripening stages were compared in order to attribute common band identities for the same band in each lane from different gel images.
The single band in each lane of the immunoblots also aligned at ∼32 KDa indicating the specificity of the antibody for UCP2 protein.
We detected only a single band in each lane of each gel, with purity > 98%.
After the lanes have been aligned, the next step is determination of band mobilities relative to the reference band in each lane.
In all animals, the presence of a modified P450 band at ∼55 kDa was prominent and represents the largest modified band in each lane.
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The number of reactive bands in each lane represents the transgene copies in each transgenic line.
Protein bands were scanned in a BioRad GS-710 Densitometer gel scanner with Quantity One software and the relative amount of proteins in stained bands in each lane were measured.
When the density of DNP-positive bands in each lane was measured, and expressed relative to GAPDH, the amount of carbonylated proteins was significantly increased (P<0.01; n = 4) by four-fold in cells treated with PINK1 siRNA, when compared to control siRNA (PINK1 #1, 6.46±1.14 relative intensity; PINK1 #2, 6.20±0.11; control siRNA, 1.61±0.21).
In addition three major bands in each lane were quantified in the Coomassie stained images.
At the end of electrophoresis, different sizes of DNA molecules appear as bands in each lane.
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