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All Western blots were quantified by measuring the band density.
The average shear band density continuously increased with plastic strain.
Band density was quantitated with ImageJ software.
Band density was measured using ImageQuant software.
Band density was quantified by TotalLab software (Durham, NC).
Equal volumes of fractions were loaded onto the gel and the band density normalised to the sum of the band density in all fractions.
Band density was normalized to the loading control and compared using the Student's t-test.
For quantification of band density, films were analyzed using densitometric software (Eagle Eye; Stratagene).
To determine band density proteins were transferred by electroblotting to Immobilon PVDF membrane (Millipore).
Band density was normalized to that of an internal control, β-actin.
The image intensities of caspas-9 and- 3 were normalized with GAPDH protein band density.
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