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The second successful case was when the flanking primers amplified a band consistent in size with the absence of the TE, and the internal primer failed to amplify any band.
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The protein bands are consistent in size to NiV proteins G, F0, F1 and M proteins [17], [48].
Also, a band appears that is consistent in size with a trimer species.
When aliquots of all proteins from 3H-EthN-labeled insoluble extracts are resolved and blotted, a 42 kDa band consistent with the size of Oto-modified Wnt3a is visible in the autoradiograph (Fig. 7a).
Increasing doses of MO1 were associated with amplification of a smaller band, consistent with the size predicted upon exclusion of exon 2 (exon 2Δ in Fig. 4B).
On the other hand, placing the pre-miRNA in the 3'-UTR of the RFP gene reduced the RFP mRNA by ~10 20% (compare lanes 1, 5 and 7 with lane 2 in Figure 3C) and resulted in a lower band (~1.5 kb, lanes 1, 5 and 7 in Figure 3C) that was consistent in size with the band expected from Drosha cropping.
Anti-ΔP recognized a ∼100-kDa band, consistent with the size of GluA4 and other AMPA receptor subunits (Figure 4B).
The asterisk marks a band consistent with the size of IgG heavy chain.
The archaeological bone points are consistent in size and breakage patterns with such uses.
PolyA-RNA hybridized with a repeat region probe revealed a 3.1 kb band consistent with the 2.95 kb size of the PNG2 ORF.
Primers were selected so that wild type band and gene trap band would differ in size.
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