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A faint band at the expected 66.7-kDa molecular weight was visible by SDS-PAGE (Fig. 4, lane 2; lane 1 presents size markers) indicating low production of the recombinant protein within the host cells.
Despite a few problems with the pointing and aspect systems that caused the telescope to track about 6.4° off the target, the analysis of the Cyg X-1 data revealed an X-ray source at 7.2σ in the 30 100 keV energy band at the expected location from the optical images taken by the onboard daytime star camera.
CB1 protein was detectable as a single band at the expected molecular mass of 51 KDa during pregnancy [32].
Western blot analyses with commercially tau-specific mAb Anti-Tau1 showed specifically reactive band at the expected position in each preparation of the eluted fraction (Fig. 2C).
Western blot analyses with GST-specific mAb identified a specific reactive band at the expected position in each preparation (Fig. 1B).
MMP9 protein in the hippocampus from cocaine users (N = 8) and control subjects (N = 8) gave a single band at the expected molecular mass of 92 kDa (Fig. 5a).
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The blots displayed bands at the expected molecular weights of ∼40 kDa and ∼47 kDa respectively by the post immune sera but not by the pre immune sera indicating a clear recognition of the endogenous EhMAPK as well as the purified recombinant EhMAPK (Fig. 1c).
All RT-PCR bands at the expected size were also directly sequenced to confirm their identity.
Denaturing electrophoresis of the purified protein reveals two bands at the expected sizes of the tagged and untagged subunits.
The blots showed the main bands at the expected size of 26 kDa for the GST protein.
Besides the bands at the expected size, other bands are as well detected by anti-pSNEV(S149) antibody on Western Blot of Hela lysates (Fig. S3), but at higher molecular weights.
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