Sentence examples for balancer line from inspiring English sources

Third, we crossed SO2A flies with a double balancer line (sp/SM5; L1/TM3, Sb) and then intercrossed the progeny (h/SM5; h/TM3 sb) to obtain h/h; h/h flies that had the 2nd and 3rd chromosomes (representing ∼80% of the genome) exclusively from the SO2A line.

To determine if any of these P-element insertions causes a mutant phenotype, each of the Top2 alleles was crossed with a CyO, Act-GFP balancer line and homozygous Top2 progeny were identified by the lack of GFP expression beginning at embryogenesis.

80 single jumpstarter males were crossed with females of the balancer line w; Dr/TM3, Sb Dfd-lacZ.

The P{Act5C-GAL4}25FO1 driver, balancer lines and the collection of EP mis-expression insertions (Rørth 1996) were obtained from the Bloomington Stock Center.

With the exception of one chromosomal inversion balancing two-thirds of the second chromosome (Benedict et al. 1999), no efficient balancer lines are yet available for A. gambiae.

The manual concludes with a section on classical strategies for the mapping of mutant alleles or transgenic constructs, including simple crosses with multiple balancer lines, deletion and meiotic mapping, and some of the complications that may arise during complementation tests (e.g., transvection, nested genes, noncoding RNA loci).

To derive the isochromosomal lines, wild-type males from each line were crossed with the Va/ Ba balancer stock [ 78], which has the same genetic background as the ch- cu strain.

To make this stock, we first crossed each insertion line to an "X-chromosome Canton-S double balancer stock" (w+; If/CyO; MKRS/TM6b) over two generations to produce w+; UAS-shibireTS1/CyO; MKRS/TM6b and w+; If/CyO; L2-Gal4/TM6b stocks.

For each founder line that yielded DsRed progeny, ∼10 individual G1 flies were crossed to the balancer stock, unless fewer flies were produced.

The balancer stock w ; T(2 3)ap Xa / CyO TM3 was used in the construction of lines and was obtained from the Bloomington Stock Center (stock 2475).

Compared to the candidate balancers, cell line-specific proteins were associated with a much broader spectrum of GO-categories (Table 4 and 5).