Sentence examples for balancer in which from inspiring English sources

Extrachromosomal arrays usually carry multiple copies of the transgene, so to test whether the aldicarb hypersensitivity of the eat-6 mutant can be corrected by a single copy of the WT gene, we took advantage of the nT1 genetic balancer, in which part of the chromosome V carrying the eat-6 locus is translocated to the chromosome IV [20].

To avoid complicating effects from mutations contained on balancer chromosomes, we have performed all experiments described below on flies in which the dFatp K10307 insertion has been backcrossed to the starter line in which the original insertion was isolated.

A similar calculation for a = 1 (see example above) is more complex if the F1 animals, in which the mutation of interest is induced on the balancer chromosome, cannot be readily distinguished from those in which the mutation is induced on the marked chromosome, and we proceed as follows.

The design problem is addressed by taking a building block approach, in which a pre-existing positive stiffness compliant grasper is compensated by a negative stiffness balancer.

And indeed, Kaplan himself concedes part of the point: he sees a world in which an "organic and united Eurasia" will demand an "organic and united North America" as a "balancer".

Thus, a pattern exists in which the development of new genetic technologies consistently leads researchers to target new transgenes to Drosophila balancer chromosomes.

The rtTA(3 E2 driver chromosome is balanced over the TM3 balancer chromosome, which is marked with the dominant mutation Sb.

After this, the first crossing task is given, in which a second and third chromosomal recessive lethal mutation need to be combined into one double-mutant stable stock using a multiple balancer stock as a further aid.

UAS-shibireTS1 and L2-Gal4 control flies were made in a similar manner, first balancing with "Canton-S single balancer stocks", which contain non-balancer chromosomes of Canton-S origin (w+; Gla/CyO; + and w+; +; MKRS/TM6b, respectively), and then crossing back to our Canton-S line to produce w+; UAS-shibireTS1/+; + and w+; +; L2-Gal4/+.

Genetic analyses are complicated by the fact that the region contains one of the largest genes in the Drosophila genome, dumpy [7], [8], alleles of which are also present on Balancer chromosomes, which means that deficiencies uncovering dumpy cannot be used in crosses with these balancers.

On two occasions, we recovered a balancer onto which the triple knockout had been recombined (the reciprocal product), which was verified by PCR, proving that the source of breakdown is in fact from the balancer (see Figure S3).