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Whereas the Wiffle has elongated holes on only one hemisphere (and therefore unpredictably rises or dips or curves), the Quickball has holes on both sides, arranged symmetrically for balanced air flow, and raised seams that allow for control over a variety of pitches.
Every well in the 96-well plate was planted 5,000 cells and incubated in a humidified 5% CO2 balanced air incubator at 37°C for 24 h.
The maximum power density increased by 15%, from 1070 ± 20 to 1230 ± 70 mW m−2, with balanced air and water pressures of 10 25 kPa.
MGC803 and GES-1 cells were cultured in a 96-well microplate at the concentration of 5,000 cells per well and incubated in a humidified 5% CO2 balanced air incubator at 37°C for 24 h.
HEK293 cells were cultured in the 96-well microplate at the concentration of 5000 cells per well and incubated in a humidified 5% CO2 balanced air incubator at 37°C for 24 h.
Gastric cancer cell line MGC803 used as target cells and human gastric mucous GES-1 used as control cells were cultured and collected[12 15], and then were treated with 50 μg/mL of prepared nanoprobes and cultured in a humidified 5% CO2 balanced air incubator at 37°C for 4 h.
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The cells were cultured and collected, then were treated with 50 μg/mL HAI-178 antibody-conjugated FMNPs nanoprobes, and cultured in a humidified 5% CO2-balanced air incubator at 37°C for 4 h.
Cells were maintained at 37°C in 5% CO2-balanced air.
The human cervical cancer cell line Hela was obtained from the American Type Culture Collection (Manassas, VA) and maintained as a monolayer in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Sijiqing Biological Engineering Materials Co., Hangzhou, China) at 37°C in the presence of 5% CO2-balanced air.
Plates were incubated in a humidified 5% CO2 balanced-air incubator at 37°C for 24, 48, and 72 h, respectively.
All cells were grown in a humidified 5% CO2 balanced-air atmosphere at 37°C.
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