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Pulmonary inflammation and damage were measured 24 hr postexposure as BAL cell counts and activity, acellular BAL albumin levels, and BAL fluid lactate dehydrogenase (LDH) activity.
No difference was found in the BAL or serum level of tumor necrosis factor-alpha or the BAL cell differential.
BAL cell types in cytospin preparations were analyzed by light microscopy and BAL supernatant was analyzed using ELISAs, specific to bovine inflammatory cytokines.
BAL cell counts started to increase by day 3 but peaked on day 6 (Fig. 5b).
Inflammation was assessed by BAL cell count, differentials and CXCL1/KC concentration.
In order to investigate the early inflammatory response following IT bleomycin, mice were studied daily for 7 days with BAL cell counts with differentials and CXCL1 levels.
Infusion of LPS in the endotoxin challenge group was also associated with an increase in the numbers of whole BAL cell counting (Fig. 1i).
After lysis of RBC, the BAL cell pellet was washed and resuspended in PBS.
These patients showed no differences in pulmonary function tests and BAL cell profile (data not shown).
At all steps in the process small aliquots were taken and cytospins performed for determination of BAL cell differential.
After OVA challenge, as expected, WT mice had significantly increased BAL cell counts compared to the PBS group.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com